Duration of the Project: 4 years

 

8.  Budget of the Project

 

1 Laboratory equipment:

 

 

Sr. No.

Equipment

Company

Units

Cost (Rs.)

1.

Laminar Air Flow

ESCO

1

2,00,000

2.

Refrigerator

Blue Star

1

50,000

3.

Minispin

Eppendorf

1

85,000

4.

Autoclave

Equitron

1

2,50,000

5.

Growth Chamber

Thermo Fisher

1

3,00,000

6.

PCR System

Eppendorf

1

5,00,000

7.

Electrophoresis Unit

Thermo Fisher

1

45,000

8.

Gel Doc

Syngene

1

2,00,00

9.

Nano Drop

Thermo Fisher

1

35,000

10.

Microwave

Life’s Good

1

10,000

11.

Magnetic stirer

Remi

1

10,000

12.

pH meter

Aczet

1

10,000

13.

Weighing balance

Scientech

1

15,000

14.

Vortex mixer

Sigma – Aldrich

1

5,000

15.

Multi-block heater

        Thermo Fisher

1

85,000

16.

Ice maker

Blue Star

1

65,000

17.

Computer

HP

1

35,000

 

Total cost of equipments: Rs. 19,00,000

 

Justification:

 

PCR mixture should be prepared under sterile condition, to avoid contamination, which clarifies the need for Laminar Air flow hood. Isolated DNA and primers need to be stored at low temperature as they are heat labile, which justifies the need for refrigerator. Minispin, Vortex, Nano drop, multi-block heater and Ice maker are needed for extraction and quantification of DNA. Few sample of plants needs to maintain in the laboratory which justifies the necessity for growth chamber. To prepare agarose gel for electrophoresis; autoclave, microwave, magnetic stirrer, pH meter and weighing balance is needed. PCR system is required to amplify the DNA. For electrophoresis and visualization of resolved gel, electrophoresis unit and gel documentation system are required. Computer would be required to analyze and store the data.

 

 

2 Manpower:

 

 

Sr. No.

Designation
 

Number of personnel

1.

Senior Scientists

1

2.

Junior Scientists

1

3.

Lab technicians

1

4.

Helper

1

 

 

Justification for Manpower and salaries /wages: –

 

Senior and Junior Scientists will be engaged in research work. Lab technician, required for

assistance in research work and to maintain the laboratory in good conditions. Helper          would be required to assist in field work.

 

The concerned institute would cater the salaries of all experts.

 

 

 

 

 

 

 

 

 

3 Consumables

 

 

Sr. No.

Item
 

Cost (Rs.)

1.

Chemicals

1,00,000

2.

DNeasy® Plant kit

2,00,000

    3.

Glass wares and Plastic wares

50,000

4.

Microfuge tubes and tips

1,00,000

5.

Miscellaneous
(Mortar and pestle, aluminum foil, cotton,Tissue roll, Gloves)

50,000

 

Total cost of consumables:  Rs. 6,00,000

 

Justification for consumables: –

The budget includes reagents for DNA extractrion, PCR mixture and electrophoresis. Majority of the chemicals will be acquired from Himedia, Sigma and SRL. The cost also includes glass wares and plastic wares which will be utilized throughout the project. Microfuge tubes and tips would be required for DNA extraction and PCR, which will be procured from Tarsons.  Miscellaneous will be purchased in bulk quantity from the local retailer.

 

 

4 Other Requirements

 

Other items

Consolidate Amount

Contingency

7,00,000

Instrumentmaintenance

3,00,000

Field *

1,00,000

 

The total cost of other requirements: Rs. 10,00,000

 

Justification

Finance required for theelectricity bill, water bill, communication bill, insurance etc. are included in contingencies. An amount is also kept aside for maintaining and validating instruments. Field amountconsists of anamountof manure, fertilizers, farming equipment etc.

* Field will be provided by the concerned institute.

 

 

Total budget of the project = Rs. 19,00,000 + 6,00,000 + 10,00,000

                                                    

                                                    = Rs. 35,00,00